Discover the structure for your RNA of interest with a personalized data package
Overview
The eSHAPE in cellulo service packages allow you to determine the secondary structure of a given transcript in the natural environment of the cell. As part of this comprehensive service, Eclipsebio’s team of experts will purchase and receive cells, perform the cell culture, process the samples, and analyze the data.
This service is offered on 3 levels, each tailored for a specific goal:
Exo | log₂(TPM)>8
Determine in cellulo structure and exogenous transfects
Standard | log₂(TPM)>6
Obtain in cellulo structure and endogenous transfects
Premium | log₂(TPM)>4
Obtain in vitro and in cellulo structure of the transcriptome, enabling the direct determination of how structure is affected by the cellular environment (∆eSHAPE). Also includes unbiased detection of transcript ends using the End-Seq service
What do you want to discover?
Exo: Discover the Structure of an Exogenous Transfect
• For genes with expression log₂(TPM)>8, perfect for transfected sequences
• Support your own analyses with raw sequencing data (FASTQ), aligned reads (BAM), and raw reactivity scores (bedGraph)
• Discover the structure of your gene of interest with a detailed, interactive report that contains quality control metrics and reactivity scores along the transcript
Standard: Deep Insights of a Single Gene
• For genes with expression log₂(TPM)>6, similar to most moderately expressed genes
• Support your own analyses with raw sequencing data (FASTQ), aligned reads (BAM), and raw reactivity scores (bedGraph)
• Discover the structure of your gene of interest with a detailed, interactive report that contains quality control metrics and reactivity scores along the transcript
Premium: Gain Full Visibility
• For genes with expression log₂(TPM)>4
• Obtain information on natural folding (in vitro), folding in the cell (in cellulo), and the identification of specific bases affected by the cellular environment (∆eSHAPE)
• Discover transcript start & end sites with End-Seq
• Support your own analyses with raw sequencing data (FASTQ), aligned reads (BAM), raw reactivity scores (bedGraph), transcript end sites (bedGraph)
