Resources
Find Eclipsebio's Data Sheets, Protocols, and Example Data Analysis Reports
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RBP-eCLIP Validated Antibodies from Eclipsebio & Cell Signaling Technology
A successful eCLIP experiment is heavily dependent on the quality of the antibody. Commercial IP-validated antibodies tend to fail validation due to eCLIP’s stringent washes. Eclipsebio & Cell Signaling Technology (CST) have partnered to expertly validate CST antibodies.
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eCLIP Pre-Validated Antibodies
Ready to start your RBP-eCLIP experiment? See Eclipsebio's full list of pre-validated antibodies
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UV Crosslinking Protocol – Tissue
See the full protocol on preparing your tissue samples
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UV Crosslinking Protocol – Adherent Cells
See the protocol for preparing your adherent cell samples
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UV Crosslinking Protocol – Suspension Cells
See the protocol for preparing your suspension cell samples
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5′ End-Seq Data Sheet
5' End-Seq facilitates active transcript end detection, enrichment and mapping, and identifies known and novel transcription start sites at single nucleotide resolution in only 2 days.
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RBP-eCLIP Data Sheet
Enhanced crosslinking and immuno-precipitation followed by high-throughput sequencing (eCLIP) was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide.
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eRibo Example Data Analysis Report
Explore your ribosome insights interactively, see the example report for eRibo Pro™
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eSHAPE db-K562 Data Sheet
eSHAPE db-K562 is a deep sequencing database containing in cellulo and in vitro SHAPE data, as well as ∆SHAPE data from the K562 cell line. The eSHAPE db-K562 is based on 6 technical replicates of polyA-selected RNA and is delivered as a data package for a single gene of interest, sets of genes, or the entire (covered) transcriptome.
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3′ End-Seq Data Sheet
3' End-Seq is a technology for defining 3' ends of transcripts by sequencing from the PAS. 3' End-Seq can identify high confidence, known and novel PAS at single nucleotide resolution in 2 days
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eSHAPE Single RNA Data Sheet
eSHAPE single RNA is a method to obtain mRNA structure probing data for in vitro transcribed mRNA formulations. In vitro transcribed mRNAs are used as input for RNA structure probing with the NAI reagent. eSHAPE single RNA is available as a kit for end user usage, and as a full service offering with results delivered as a data package containing RNA structure information as mutations and reactivities.