of the RBP-RNA Interactome
RNA binding proteins (RBPs) bind to RNAs through recognition of sequence and structural motifs to regulate RNA function in a cell-type, condition-specific, or temporal manner. Recent studies have estimated that there are over 1500 RBPs in the human genome and these RBPs play an integral role modulating RNA stability and function throughout the RNA life cycle. Mutations in RBPs have been linked to cancer, Amyotrophic Lateral Sclerosis (ALS), and numerous other diseases.
Enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP) was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide. Eclipse Bio has optimized the eCLIP technology developed at UCSD and described in the 2016 Nature Methods paper to improve the efficiency of converting immunoprecipitated RNA into high-throughput sequencing libraries.
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