RBP-eCLIP
High resolution maps
of the RBP-RNA Interactome
of the RBP-RNA Interactome
Technology Overview
RNA binding proteins (RBPs) bind to RNAs through recognition of sequence and structural motifs to regulate RNA function in a cell-type, condition-specific, or temporal manner. Recent studies have estimated that there are over 1500 RBPs in the human genome and these RBPs play an integral role modulating RNA stability and function throughout the RNA life cycle. Mutations in RBPs have been linked to cancer, Amyotrophic Lateral Sclerosis (ALS), and numerous other diseases.
Enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP) was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide. Eclipse Bio has optimized the eCLIP technology developed at UCSD and described in the 2016 Nature Methods paper to improve the efficiency of converting immunoprecipitated RNA into high-throughput sequencing libraries.
Technology Highlights
High Efficiency Library Preparation with Decreased PCR Duplication
- Altered enzymatic steps improved library preparation by 1000-fold compared to other CLIP methods
- RBP-eCLIP increases experimental success rates and decreases wasted sequencing due to PCR duplication
Click Image to enlarge
Click Image to enlarge
Transcriptome-wide
Identification of RNA Targets
- RBP-eCLIP identifies RBP binding sites across all genic regions: exons, introns and UTRs
- RBP-eCLIP reveals coding and non-coding RBP targets, including lincRNAs, microRNAs, and retrotransposons
RBP RNA binding motif identification
- RBP-eCLIP can be used to validate known RNA binding motifs
- Discover novel RNA binding motifs with RBP-eCLIP
Click Image to enlarge
RESOURCES
Services Tailored to Your Experiment
We offer all-inclusive sample processing with expert library preparation and bioinformatic analysis.
