Technology Overview
m6A-eCLIP™, also known as methylated RNA crosslinking and immunoprecipitation, is a cutting-edge technique allowing researchers to identify m6A-modifications, transcriptome-wide, with single nucleotide resolution, thus providing the ability to elucidate the functional consequences of m6A in gene regulation.
N6-methyladenosine (m6A) is the most prevalent RNA modification found in eukaryotes. m6A modification has been shown to regulate many aspects of RNA biology including splicing, secondary and tertiary structure, nuclear export, localization, stability, and translation. Levels of m6A modification at specific positions is tuned by writer and eraser enzymes and has been shown to play a role in stem cell renewal and differentiation, and dysregulation of m6A states has been implicated in a wide variety of cancers
m6A-eCLIP enables researchers to perform integrative analyses with other genomic data such as RBP-eCLIP to gain a comprehensive understanding of RNA regulatory mechanisms. The information obtained from m6A-eCLIP studies contributes to the development of targeted therapies and the discovery of biomarkers for diseases such as cancer, neurological disorders, and metabolic disorders.