High Resolution Maps
of the Epitranscriptome
N6-methyladenosine (m6A) is the most prevalent RNA modification found in eukaryotes. m6A modification has been shown to regulate many aspects of RNA biology including splicing, secondary and tertiary structure, nuclear export, localization, stability, and translation. Levels of m6A modification at specific positions is tuned by writer and eraser enzymes and has been shown to play a role in stem cell renewal and differentiation, and dysregulation of m6A states has been implicated in a wide variety of cancers.
m6A-eCLIP robustly profiles m6A modification sites with single nucleotide resolution transcriptome-wide. In comparison to alternate approaches, m6A-eCLIP requires 100-fold less starting RNA material for m6A profiling. m6A-eCLIP identifies over 70,000 enriched modification sites per sample, of which more than 70% are positioned directly at adenosines and more than 50% are positioned at adenosines in the context of the DRACH motif, a well characterized 5-nucleotide (nt) motif preferred by m6A methyltransferases (the DRACH motif uses IUPAC nucleotide codes where D represents an A, G or U nucleotide, R represents an A or a G nucleotide, and H represents an A, C or U nucleotide).