Services

Discover new insights with our end-to end genomics solutions

RNA Genomics Services

Eclipse Bio offers a wide portfolio of RNA services to support existing and emerging techniques in the RNA field. Whether you are conducting exploratory research projects, investigating new RNA drug targets, or comparing RNA data sets, we offer RNA genomics solutions for your needs.

Established Services Offered

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RBP-eCLIP

m6A-eCLIP

miR-eCLIP

5' & 3' End-Seq

RBP-eCLIP

RNA binding proteins (RBPs) bind to RNAs through recognition of sequence and structural motifs to regulate RNA function in a cell-type, condition-specific, or temporal manner. Enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP) was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide.

Read more about RBP-eCLIP

Antibody

Starting Material

CELLS
TISSUE

Turn Around

Samples ≥ 2 = 4-6 weeks

Samples ≤ 2 = 6-8 weeks

Expedited available upon request.

m6A-eCLIP

N6-methyladenosine (m6A) is the most prevalent RNA modification found in eukaryotes. m6A-eCLIP robustly profiles m6A modification sites with single nucleotide resolution transcriptome-wide.

Read more about m6A-eCLIP

Antibody

Starting Material

RNA

Turn Around

Samples ≥ 2 = 4-6 weeks

Samples ≤ 2 = 6-8 weeks

Expedited available upon request.

miR-eCLIP

Precise microRNA (miRNA) target identification is essential to understand post-transcriptional gene regulation. The miR-eCLIP technology enables precise mapping of direct miRNA-mRNA interactions transcriptome wide. miR-eCLIP also has the option of enriching for specific miRNAs or genes of interest, enabling profiling of miRNA-target interactions at an unprecedented depth.

Read more about miR-eCLIP

Antibody

Starting Material

CELLS
TISSUE

Turn Around

Samples ≥ 2 = 4-6 weeks*

Samples ≤ 2 = 6-8 weeks*

*Additional 1-2 weeks with enrichments.

Expedited available upon request.

Enrichment Additional Specifications

+ Gene

Enrich to identify miRNA target sites for all expressed miRNAs binding a gene of interest.

+ miR

Enrich miRNA target sites for miRNA(s) of interest transcriptome wide.

5' & 3' End-Seq

The End-Seq technology enables mapping of the 5′ ends and 3′ ends of transcripts by sequencing from the TSS and PAS, respectively, revealing the complete UTR landscape of expressed transcripts. 5′ and 3′ End-Seq enrich for transcript ends to detect known and novel TSS and PAS at single nucleotide resolution.

Read more about End-Seq

Starting Material

RNA

Turn Around

Samples ≤ 12 = 4-6 weeks

Expedited available upon request.

Custom options available upon request. 

Data Analysis Deliverables

Data Files RBP-eCLIP m6A-eCLIP miR-eCLIP 5' End-Seq 3' End-Seq
Raw FASTQ: Reads directly off sequencer, demultiplexed per sample
Trimmed FASTQ: Reads after UMI and 3' adapter trimming
Alignment BAM: Reads filtered of repetitive elements, aligned, and PCR deduplicated
Alignment BAM Index: Index file corresponding to above alignment BAM
Coverage BigWig: Normalized read coverage on positive and negative strands
Peaks BED: Input normalized read clusters with log2 enrichment score
Single Nucleotide Coverage Bedgraph: 5’ end read coverage
Single Nucleotide Calls BED: Single base modification sites or end calls
Filtered Single Nucleotide Calls: Single base modification sites or end calls after filtering
miRNA Read Alignment TSV: Position of the best aligned miRNA in miR-mRNA reads
miRNA-mRNA targets BED: Genomic mRNA locations of miR-mRNA reads
Report HTML: Report with tables and plots for enriched GO terms, KEGG pathways, motifs, and repetitive elements for enriched peaks