RNA Genomics Services
Eclipsebio offers a wide portfolio of RNA services to support existing and emerging techniques in the RNA field. Whether you are conducting exploratory research projects, investigating new RNA drug targets, or comparing RNA data sets, we offer RNA genomics solutions for your needs.
Established Services Offered
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RBP-eCLIP
m6A-eCLIP
miR-eCLIP
5' & 3' End-Seq
RBP-eCLIP
RNA binding proteins (RBPs) bind to RNAs through recognition of sequence and structural motifs to regulate RNA function in a cell-type, condition-specific, or temporal manner. Enhanced crosslinking and immunoprecipitation followed by high-throughput sequencing (eCLIP) was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide.
Starting Material
CELLS
- 20 M
- UV Crosslinked
- Mouse, Human
TISSUE
- 80 mg
- NOT Crosslinked
- Human, Mouse, Rat, C. elegans
Turn Around
Samples ≥ 2 = 6-8 weeks
Samples ≤ 2 = 8-10 weeks
Expedited available upon request.
m6A-eCLIP
N6-methyladenosine (m6A) is the most prevalent RNA modification found in eukaryotes. m6A-eCLIP robustly profiles m6A modification sites with single nucleotide resolution transcriptome-wide.
Antibody
- Eclipsebio provided m6A antibody
Starting Material
RNA
- 20 µg
- RNA Integrity ≥ 7
- NOT Crosslinked
- Human, Mouse, Rat
Turn Around
Samples ≥ 2 = 6-8 weeks
Samples ≤ 2 = 8-10 weeks
Expedited available upon request.
miR-eCLIP
Precise microRNA (miRNA) target identification is essential to understand post-transcriptional gene regulation. The miR-eCLIP technology enables precise mapping of direct miRNA-mRNA interactions transcriptome wide. miR-eCLIP also has the option of enriching for specific miRNAs or genes of interest, enabling profiling of miRNA-target interactions at an unprecedented depth.
Starting Material
CELLS
- 20 M
- UV Crosslinked
- Mouse, Human
TISSUE
- 80 mg
- NOT Crosslinked
- Human, Mouse
Turn Around
Samples ≥ 2 = 6-8 weeks*
Samples ≤ 2 = 8-10 weeks*
Expedited available upon request.
Enrichment Additional Specifications
+ Gene
Enrich to identify miRNA target sites for all expressed miRNAs binding a gene of interest.
- Customer provides Gene name
- Eclipsebio generates probe
- Custom Project - Contact Us
+ miR
Enrich miRNA target sites for miRNA(s) of interest transcriptome wide.
- Customer provides miRNA name
- Eclipsebio generates probe
5' & 3' End-Seq
The End-Seq technology enables mapping of the 5′ ends and 3′ ends of transcripts by sequencing from the TSS and PAS, respectively, revealing the complete UTR landscape of expressed transcripts. 5′ and 3′ End-Seq enrich for transcript ends to detect known and novel TSS and PAS at single nucleotide resolution.
Starting Material
RNA
- 6 µg
- RNA Integrity ≥ 7
- NOT Crosslinked
Turn Around
Samples ≤ 12 = 4-6 weeks
Expedited available upon request.
Custom options available upon request.