A Ribosome Profiling Service
eRibo Pro (RiboSeq)
High resolution maps of ribosome footprints
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Technology Overview
eRibo Pro™ is an exclusively licensed method for profiling ribosome-associated RNAs and the transcriptome. The eRibo Pro ribosome profiling service is an end-to-end solution that streamlines the ribosome profiling process, from sample processing to sequencing and data analysis.
Widespread application of RNA-Seq has provided substantial insight into the role of gene expression. However it fails to capture regulation at the level of translation. Furthermore, while many people utilize RNA-Seq data to estimate the cellular proteome characterization of ribosome-associated RNAs serves as a closer proxy to estimate a sample’s protein levels.
This comprehensive service includes mapping of ribosome protected fragments (RPFs) and identification of differentially transcribed and translated genes with interactive reports that allow researchers to make data driven insights. The ability to quantify ribosome occupancy transcriptome-wide using eRibo Pro will enable translatome studies to be applied more broadly in the context of research and in the development of therapeutics.
Eclipsebio is sole licensee of the Ribo-seq method covered by U.S. Patent Numbers 8,486,865, 9,284,547 and 9,677,068.
Technology Highlights
Technology Highlights
Detect changes in ribosome occupancy
- Receive two data sets from one sample: a ribosome protected fragment (RPF) library and an RNA-Seq library
- Quantify changes in transcription and translation in response to therapeutic treatments
High quality ribosome profiling data
- RPF libraries capture expected trinucleotide periodicity from translating ribosomes
- eRibo Pro delivers high resolution ribosome binding information
High correlation between eRibo replicates
- eRibo Pro provides high correlation between RPF, RNA-Seq, and RO datasets
- Robust and reproducible ribosome profiling data provides high confidence to assess translational efficiency
Detect changes in ribosome occupancy
- Receive two data sets from one sample: a ribosome protected fragment (RPF) library and an RNA-Seq library
- Quantify changes in transcription and translation in response to therapeutic treatments
High quality ribosome profiling data
- RPF libraries capture expected trinucleotide periodicity from translating ribosomes
- eRibo Pro delivers high resolution ribosome binding information
