All-Inclusive Service
5′ End-Seq
Reveal 5′ UTR Landscape and Transcription Start Sites Genome-wide
Contact UsThe 5′ End-Seq Service
Turnaround Time
Service: 6 – 8 Weeks (Project Dependent)
Starting Material
10 µg Total RNA; RIN ≥ 7; Non-Crosslinked
Minimum Batch: 8 Samples
Start your 5′ End-Seq Service Project
Connect With An Eclipsebio RNA Expert
Bioinformatics
Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), rat (rn6), or c. elegans (ce11). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis
COVERAGE TRACKS | BIGWIG
Normalized read end coverage on positive and negative strands for visualization in a genome viewer such as IGV
SINGLE NUCLEOTIDE 5′ END CALLS | BED
Genomic single nucleotide positions scored as putative 5’ transcript ends for 5’UTR exploration
5′ End-Seq SUMMARY REPORT | HTML
Report with interactive tables and plots for publication ready graphics. Includes QC metrics and 5’ end enrichment