Nanopore sequencing measures RNA quality with long-read sequencing. Learn how below.
Nanopore RNA sequencing with Oxford Nanopore Technologies (ONT) provides long-read data to detect modifications and impurities that impact therapeutic safety and performance. This direct sequencing approach enables comprehensive RNA quality assessments, supporting drug developers in optimizing manufacturing and ensuring consistent, high-quality RNA therapeutics.
Contact usOverview
Use:
Direct RNA characterization
Typical species:
Viral, human
Typical samples:
IVT RNA
Characterize with direct sequencing
Ready to start your nanopore sequencing project? Contact us today.
Contact usPrepare libraries
Sequencing libraries can be prepared directly from the RNA or cDNA
Sequencing
Long-read, nanopore-based sequencing is performed
Analyze
Custom bioinformatics analyses are performed to assess RNA quality
RNA characterization with long-read sequencing
The following are some examples of the types of insights that nanopore sequencing can provide. Different analyses can be performed based on our partner's needs.
Measure poly(A) tail length
Nanopore RNA sequencing enables direct measurement of poly(A) tail lengths on full-length transcripts, providing insight into a key driver of RNA therapeutic performance.
Poly(A) tail length distribution of an in vitro transcribed RNA with a templated poly(A) tail. The lengths cluster around the expected 120 nucleotides.
Map RNA fragments
Nanopore sequencing enables direct detection and characterization of RNA fragments, providing valuable insight into RNA integrity and stability. This information helps drug developers monitor RNA quality during production and storage, and optimize formulations to improve the stability and performance of RNA therapeutics.
Nanopore sequencing detects RNA fragments of varying lengths added to a full-length IVT RNA, demonstrating sensitive detection of tailed RNA fragments.