Reveal integrity, identity, and poly(A) tail length in a single experiment
Nanopore RNA sequencing with Oxford Nanopore Technologies (ONT) provides long-read data to detect modifications and impurities that impact therapeutic safety and performance. This direct sequencing approach enables comprehensive RNA quality assessments, supporting drug developers in optimizing manufacturing and ensuring consistent, high-quality RNA therapeutics.
Contact usOverview
Use:
Direct RNA characterization
Typical species:
Viral, human
Typical samples:
IVT RNA
Characterize with direct sequencing
Ready to start your nanopore sequencing project? Contact us today.
Contact usPrepare libraries
Sequencing libraries can be prepared directly from the RNA or cDNA
Sequencing
Long-read, nanopore-based sequencing is performed
Analyze
Custom bioinformatics analyses are performed to assess RNA quality
RNA characterization with long-read sequencing
The following are some examples of the types of insights that nanopore sequencing can provide. Different analyses can be performed based on our partners' needs.
Measure poly(A) tail length with precision
Nanopore RNA sequencing directly measures poly(A) tail lengths on IVT RNAs, providing insight into a key determinant of stability, translation efficiency, and overall therapeutic performance.
Poly(A) tail length distributions from an RNA synthesized by two manufacturers. One manufacturer (teal) produced tails clustered at the expected length, while the other (orange) showed variable tails associated with reduced protein output.
Verify RNA identity with single-nucleotide resolution
Direct RNA sequencing enables base-by-base measurement of an IVT RNA without cDNA conversion. By examining each incorporated base in its native form, drug developers can detect misincorporation events to identify plasmid or polymerase issues that impact RNA quality.
Nanopore sequencing of an IVT RNA showing the percentage of molecules carrying the expected guanine at each position. The highlighted site represents a misincorporation event where adenine occurred 94% of the time.
Profile full-length RNA transcript isoforms
Long-read nanopore sequencing captures complete RNA transcripts, enabling accurate assessment of isoform diversity in complex transcriptomes. By resolving full-length molecules without assembly, developers can quantify how many transcripts match the expected reference sequence and identify novel, extended, or truncated isoforms that may influence RNA function or therapeutic behavior.
Distribution of transcript isoform types detected in HepG2 using nanopore sequencing. Most transcripts matched the expected reference, while a subset represented novel, extended, or truncated isoforms that highlight transcriptome complexity.