eSENSE m6A maps methylated regions transcriptome-wide from low levels of input material, providing detailed information to better understand RNA biology and develop more effective RNA therapeutics.
Contact useSENSE m6A maps regions of RNA methylation transcriptome-wide. Learn how below.
N6-methyladenosine (m6A) is one of the most prevalent RNA modifications and has been implicated in key aspects of RNA biology including RNA structure, stability and translation. Our eSENSE m6A technology is an enhanced version of MeRIP-Seq that provides the detection of methylated RNA regions with ultra-low input requirements (down to 2 μg total RNA).
Contact usOverview
Use:
Map methylated regions
Typical species:
Human, mouse
Typical samples:
Total RNA
Locate methylated regions
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Contact usImmunoprecipitate
A selective antibody is used to immunoprecipitate methylated regions from low levels of input RNA.
Sequence
Libraries are prepared to provide region-level identification of methylated bases.
Analyze
Custom bioinformatics analyses map methylated regions.
Methylation revealed: eSENSE m6A and
m6A-eCLIP
RNA methylation is a critical regulator of disease and therapeutic efficacy. To support the needs of our partners, we offer two complementary assays that reveal RNA methylation at different scales.
m6A-eCLIP is derived from our eCLIP-family of assays. It detects both large methylated regions and the specific bases that are methylated in a given region.
eSENSE m6A is our optimized version of MeRIP-Seq. It detects large regions of methylation, but the specific methylated bases are not identified. eSENSE m6A has higher throughput than m6A-eCLIP, allowing researchers to screen more samples in a single experiment. eSENSE m6A also detects methylation with lower input amounts (down to 2 μg total RNA) than m6A-eCLIP.
No matter what resolution you need to examine RNA methylation, our assays provide you with the data you need.
Discover regions of RNA methylation
The following are some examples of the types of insights that eSENSE m6A can provide. For our partners, we perform additional analyses to provide actionable insights for therapeutic success.
Locate regions of RNA methylation
Like traditional MeRIP-Seq, eSENSE m6A maps regions of elevated methylation across expressed genes with low input requirements. This provides researchers and drug developers with critical information on where RNA modifications are deposited across the transcriptome. For increased resolution, our m6A-eCLIP assay provides base-level mapping of methylation.
Gene browser tracks showing methylated regions from eSENSE m6A, Eclipsebio's enhanced version of MeRIP-Seq, compared to m6A-eCLIP. eSENSE m6A provides region-level information on RNA methylation while m6A-eCLIP identifies specific methylated sites.
Identify classes of methylated genes
For transcriptome-wide studies of methylation, we perform gene ontology analyses to determine if specific categories of genes are preferentially methylated in a given disease state or after a therapeutic treatment.
Word cloud showing categories of genes that have increased methylation.
Find methylated gene features
eSENSE m6A provides detailed information on methylated RNA regions, such as which specific genes have increased methylation at stop codons or UTRs. As part of our standard analyses for eSENSE m6A we identify which gene features are methylated, providing researchers with key insights into how methylation patterns change with specific disease or drug treatments.
Line plot showing the expected enrichment of RNA methylation at the stop codon and 3' UTR of expressed genes. The reproducible line indicates peaks of RNA methylation that are statistically reproducible among both replicates.