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Discover where and how miRNAs bind with miR-eCLIP
By controlling RNA stability and translation, miRNAs are key regulators of RNA biology. Our miR-eCLIP technology uses direct immunoprecipitation of RISC and chemical ligation of miRNAs to their target mRNAs to map miRNA binding across the transcriptome.
We have used our miR-eCLIP assay to map the miRNA regulatory landscape of the K562 cell line. Jumpstart your therapeutic development or discover new aspects of RNA biology with this deeply sequenced dataset.
Technology:
miR-eCLIP
Sample:
K562 cell line
Use:
Uncover the regulatory network of druggable targets
Complete data, comprehensive analyses
This dataset includes aligned sequencing data to support your own discoveries as well as analysis performed by our internal bioinformatics team to reveal key insights of miRNA behavior.
Explore the data with interactive reports
To get to actionable insights faster, we have generated an interactive report to assist with data exploration. Included in this report are plots that reveal canonical and noncanonical binding by miRNAs, miRNA abundance, and the categories of genes are being regulated by miRNAs.
An example of our miR-eCLIP report, which provides interactive plots and searchable tables.
Visualize miRNA activity
We provide bigWig tracks to visualize where chimeric miRNA-mRNA reads are accumulating and BED files that list miRNA binding positions. With these files, researchers and drug developers can examine miRNA binding across genes of interest or zoom out to see patterns of miRNA regulation across the transcriptome.
Direct detection of miRNA binding in the 3' UTR of E2F3. Each row is a different miRNA. Peaks are where that specific miRNA binds.
Make your own discoveries
BAM files contain information on where each miRNA-mRNA chimeric read maps across the genome. This file can be used by bioinformaticians to perform analyses for specific research and therapeutic goals. The raw data can also be used as input to train machine learning models to predict miRNA activity.
The miR-eCLIP dataset can be used to make new discoveries and train models for therapeutic design.