The miR-eCLIP Service
6-8 Weeks (+2 Weeks for Enrichment)
Eclipsebio provided AGO2 antibody
Reactive in human, mouse, rat
Cells: 20 M; UV Crosslinked
Tissue: 80 mg; Non-Crosslinked; Flash Frozen
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Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Unique AGO2 eCLIP sequence alignments to the genome. Reads aligned to repetitive elements were filtered out and PCR duplicates were removed.
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
AGO2, miRNA, & siRNA PEAKS | BED
Table listing the binding locations and relative enrichment of siRNAs and miRNAs
TARGET SUMMARY REPORT | HTML
HTML report containing plots, enriched GO terms and KEGG pathways, HOMER motif analysis, and repetitive element mapping information for enriched peaks.