Services + Kits
miR-eCLIP
Precise mapping of direct miRNA-mRNA interactions transcriptome wide
Contact Us
The miR-eCLIP Service
Turnaround Time
6-8 Weeks (+2 Weeks for Enrichment)
Antibody
Eclipsebio provided AGO2 antibody
Reactive in human, mouse, rat
Starting Material
Cells: 20 M; UV Crosslinked
Tissue: 80 mg; Non-Crosslinked; Flash Frozen
Start your miR-eCLIP Service Project
Connect With An Eclipsebio RNA Expert
Bioinformatics
Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), and rat (rn6). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis, with AGO2 non chimeric reads and miRNA target chimeric reads in two separate files
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
SCORED AGO2 & miRNA TARGET PEAKS | BED
Genomic regions of putative AGO2 binding sites or miRNA target sites (aka “peaks”) for target exploration. Includes log2 enrichment scores and p-values after input normalization for AGO2 peaks or read count and miRNA name for miRNA target peaks
miRNA TARGET SUMMARY REPORT | HTML
Report for enriched AGO2 and miRNA target peaks with interactive tables and plots for publication ready graphics. Includes seed match analysis, miRNA abundance analysis, differential miRNA target analysis (if applicable), enriched GO terms, KEGG pathways, and motifs. See Example Data Analysis Report
