All-Inclusive Service
miR-eCLIP
Precise mapping of direct miRNA-mRNA interactions transcriptome wide
Contact Us
The miR-eCLIP Service
Turnaround Time
6-8 Weeks (+2 Weeks for Enrichment)
Antibody
Eclipsebio provided AGO2 antibody
Reactive in human, mouse, rat
Starting Material
Cells: 20 M; UV Crosslinked
Tissue: 80 mg; Non-Crosslinked; Flash Frozen
Start your miR-eCLIP Service Project
Connect With An Eclipsebio RNA Expert
Bioinformatics
Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Unique AGO2 eCLIP sequence alignments to the genome. Reads aligned to repetitive elements were filtered out and PCR duplicates were removed.
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
AGO2, miRNA, & siRNA PEAKS | BED
Table listing the binding locations and relative enrichment of siRNAs and miRNAs
TARGET SUMMARY REPORT | HTML
HTML report containing plots, enriched GO terms and KEGG pathways, HOMER motif analysis, and repetitive element mapping information for enriched peaks.
