All-Inclusive Service
m6A-eCLIP
A robust approach to profile m6A modification sites at single nucleotide resolution, transcriptome-wide
Contact Us
The m6A-eCLIP Service
Turnaround Time
6-8 Weeks (Project Dependent)
Antibody
Eclipsebio provided m6A antibody
Starting Material
50ug Total RNA; RIN >7
Isolated from Cells or Tissues
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Bioinformatics
Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), rat (rn6), or c. elegans (ce11). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
SCORED PEAKS & SITES | BED
Genomic regions (aka “peaks”) and single nucleotide positions scored after input normalization as putative m6A sites for target exploration. Includes log2 enrichment scores and p-values
m6A SUMMARY REPORT | HTML
Report for enriched peaks and sites with interactive tables and plots for publication ready graphics. Includes enriched GO terms, KEGG pathways, motifs, and repetitive element analysis