All-Inclusive Service

m6A-eCLIP

A robust approach to profile m6A modification sites at single nucleotide resolution, transcriptome-wide

The m6A-eCLIP Service

Turnaround Time
6-8 Weeks (Project Dependent)

Antibody
Eclipsebio provided m6A antibody

Starting Material
50ug Total RNA; RIN >7
Isolated from Cells or Tissues

Start your m6A-eCLIP Service Project
Connect With An Eclipsebio RNA Expert

Bioinformatics

Our Comprehensive Data Analysis Package

Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), rat (rn6), or c. elegans (ce11). Contact us for alternate species including viral genomes.

EXAMPLE REPORT

RAW SEQUENCING DATA | FASTQ

Reads directly off sequencer and demultiplexed per sample for data back-up and publication

GENOME ALIGNMENTS | BAM

Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis

COVERAGE TRACKS | BIGWIG

Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV

SCORED PEAKS & SITES | BED

Genomic regions (aka “peaks”) and single nucleotide positions scored after input normalization as putative m6A sites for target exploration. Includes log2 enrichment scores and p-values

m6A SUMMARY REPORT | HTML

Report for enriched peaks and sites with interactive tables and plots for publication ready graphics. Includes enriched GO terms, KEGG pathways, motifs, and repetitive element analysis