All-Inclusive Service

eSHAPE Single RNA

Secondary structure of RNA molecules

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The eSHAPE Service

Turnaround Time
Service: 4 – 6 Weeks (Project Dependent)

Starting Material
1ug in vitro transcribed RNA

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Bioinformatics

Our Comprehensive Data Analysis Package

RAW SEQUENCING DATA | FASTQ

Reads directly off sequencer and demultiplexed per sample for data back-up and publication

RNA ALIGNMENTS | BAM

Reads aligned to the RNA of interest, and PCR deduplicated for additional downstream analysis

MUTATION RATES | TSV

Mutation rates for each NAI and DMSO sample per RNA position for reactivity calculations

eSHAPE REACTIVITY | BEDGRAPH, SHAPE

IQR normalized reactivity score at each position of the RNA of interest with >1000x coverage for visualization in a genome viewer such as IGV or for use as input into the RNAStructure algorithm to guide fold predictions

eSHAPE SUMMARY REPORT | HTML

Report of eSHAPE results with interactive tables and plots for publication ready graphics. Includes QC metrics, coverage, mutation rates and reactivities for the RNA of interest

REACTIVITY COMPARISON REPORT | HTML

Report of differential reactivities for experiments with multiple conditions or RNAs for comparison. Includes interactive plots and results table denoting significantly different positions with p-values

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