Products
RBP-eCLIP
RBP-eCLIP (Enhanced crosslinking and immunoprecipitation, eCLIP) followed by high-throughput sequencing was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide.
Service Available
Kit Available
Turnaround time
Service: 6-8 Weeks (Project Dependent)
Antibody
- *Requires Validated Antibody
- See List of Pre-Validated Antibodies
- Antibody Validation Services Available
Starting Material
- Cells: 20 M; UV Crosslinked
Tissue: 80 mg; NOT Crosslinked; Flash Frozen
miR-eCLIP
miR-eCLIP enables precise mapping of direct miRNA-mRNA interactions transcriptome wide. In contrast to alternative techniques that provide indirect methods to map miRNA targets (i.e., computational algorithms or over-expression/knockdown experiments), miR-eCLIP identifies direct in vivo miRNA targets using AGO2 immunoprecipitation, RNA-RNA ligation, and high-throughput sequencing (similar to methods such as CLASH or CLEAR-CLIP).
Service Available
Kit Not Available
Turnaround time
6-8 Weeks (Project Dependent)
+2 Weeks for Enrichment
Antibody
- Eclipsebio provided AGO2 antibody
Starting Material
- Cells: 20 M; UV Crosslinked; Mouse, Human
Tissue: 80 mg; NOT Crosslinked; Human, Mouse, Rat, C. elegans
m6A-eCLIP
m6A-eCLIP robustly profiles m6A modification sites with single nucleotide resolution transcriptome-wide. In comparison to alternate approaches, m6A-eCLIP requires 100-fold less starting RNA material for m6A profiling.
Service Available
Kit Available
Turnaround time
Service: 6-8 Weeks (Project Dependent)
Antibody
- Eclipsebio provided m6A antibody
Starting Material
- 50ug Total RNA; RIN >7
Isolated from Cells or Tissues
eRibo Pro
eRibo Pro is a proprietary ribosome profiling service that streamlines the ribosomal profiling process, from sample processing to sequencing and data analysis. The comprehensive service includes mapping of ribosome protected fragments (RPFs) and identification of differentiallv transcribed and translated genes with interactive reports that allow researchers to make data driven insights.
Eclipsebio is sole licensee of the Ribo-seq method covered by U.S. Patent Numbers 8,486,865, 9,284,547 and 9,677,068.
Service Available
Kit Not Available
Turnaround time
Service: 6 – 8 Weeks (Project Dependent)
Starting Material
- 5M Cells
- Starting from 70% – 80% Confluent Plates
eSHAPE Single RNA
eSHAPE is a proprietary method to obtain RNA structure probing data for in vitro transcribed RNA formulations. In vitro transcribed or synthetic RNAs are used as input for RNA structure probing with the NAI reagent. eSHAPE single RNA is available as a kit for end user completion, and as a full service offering with results delivered as a data package containing RNA structure information as reactivity values.
Service Available
Kit Available
Turnaround time
Service: 6 – 8 Weeks (Project Dependent)
Starting Material
- 1ug in vitro transcribed RNA
5′ End-Seq
5′ End-Seq can facilitate precise UTR identification for RNA therapeutics targeting and can be used as a tool for identifying UTR biomarkers associated with disease. 5′ End-Seq can quantify the relative usage of TSS across samples and indicates transcript isoform presence in annotated transcriptomes. Defining the 5′ UTR with End-Seq can help predict binding factor motifs more reliably
Service Available
Kit Available
Turnaround time
Service: 4 – 6 Weeks (Project Dependent)
Starting Material
- 10 µg Total RNA; RIN ≥ 7; Non-Crosslinked
- Minimum Batch: 8 Samples
3′ End-Seq
3′ End-Seq can facilitate precise UTR identification for RNA therapeutics targeting and can be used as a tool for identifying UTR biomarkers associated with disease. 3′ End-Seq can quantify the relative usage of PAS across samples and indicate transcript isoform presence in annotated transcriptomes. Defining the 3′ UTR with End-Seq helps predict binding factor motifs more reliably.
Service Available
Kit Available
Turnaround time
Service: 4 – 6 Weeks (Project Dependent)
Starting Material
- 10 µg Total RNA; RIN ≥ 3; Non-Crosslinked
- Minimum Batch: 8 Samples
