m6A-eCLIP maps methylated bases transcriptome-wide, providing detailed information to better understand RNA biology and develop more effective RNA therapeutics.
Contact usm6A-eCLIP determines which exact bases are methylated, revealing the dynamics of RNA modifications.
N6-methyladenosine (m6A) is one of the most prevalent RNA modifications and has been implicated in key aspects of RNA biology, including RNA structure, stability and translation. Unlike traditional approaches for studying RNA methylation that only map regions with RNA methylation, the m6A-eCLIP technology provides single nucleotide resolution of methylated sites across the transcriptome and on transfected sequences.
Contact usOverview
Use:
Map methylated bases
Typical species:
Human, mouse
Typical samples:
Total RNA
Locate specific methylated bases transcriptome-wide
Ready to start your m6A-eCLIP project? Contact us today.
Contact usImmunoprecipitate
A selective antibody is used to immunoprecipitate methylated RNA
Sequence
Libraries are prepared to provide base-level identification of methylated bases
Analyze
Custom bioinformatics analyses map specific methylated bases
Methylation revealed: m6A-eCLIP and
eSENSE m6A
RNA methylation is a critical regulator of disease and therapeutic efficacy. To support the needs of our partners, we offer two complementary assays that reveal RNA methylation at different scales.
m6A-eCLIP is derived from our eCLIP-family of assays. It detects both large methylated regions and the specific bases that are methylated in a given region.
eSENSE m6A is our optimized version of MeRIP-Seq. It detects large regions of methylation, but the specific methylated bases are not identified. eSENSE m6A has higher throughput than m6A-eCLIP, allowing researchers to screen more samples in a single experiment. eSENSE m6A also detects methylation with lower input amounts (down to 2 μg total RNA) than m6A-eCLIP.
No matter what resolution you need to examine RNA methylation, our assays provide you with the data you need.
Discover methylated bases
The following are some examples of the types of insights that m6A-eCLIP can provide. For our partners, we perform additional analyses to provide actionable insights for therapeutic success.
Site-specific identification of methylation
m6A-eCLIP reveals methylation patterns across multiple scales: from identifying regions where methylation is elevated (similar to MeRIP-Seq) to providing identification of which specific adenosines are methylated. This provides researchers and drug developers with an unparalleled view into where RNA modifications are deposited across the transcriptome and transfected sequences.
Gene browser tracks showing methylated regions from m6A-eCLIP compared to eSENSE m6A, Eclipsebio's enhanced version of MeRIP-Seq. Site-specific methylated adenosines show high concordance with expected DRACH motifs.
Identify classes of methylated genes
For transcriptome-wide studies of methylation, we perform gene ontology analyses to determine if specific categories of genes are preferentially methylated in a given disease state or after a therapeutic treatment.
Word cloud showing categories of genes that have methylated bases.
Locate differential methylation
m6A-eCLIP provides per-base and per-region measurements of methylation. This allows us to examine changes in methylation on multiple scales, providing deeper insights into how a condition or drug treatment affects methylation patterns. This type of analysis can be used to confirm if a therapeutic has changed methylation at a specific target and if off-target changes are occurring.
Scatter plot of individual adenosines across the genome. Differentially methylated bases are highlighted in orange and teal.