Ribo-eCLIP

Simplified approach to reveal ribosome occupancy transcriptome-wide

Technology Overview

Determining the composition of the proteome and relative abundance of proteins in biological and clinical samples is a fundamental component of biomedical research. Methodologies that quantitate transcripts that are associated with ribosomes and actively being translated yield a more accurate and unbiased representation of protein levels; however, current strategies are laborious.

Ribo-eCLIP is a method that maps and quantifies ribosome occupancy transcriptome-wide by targeting a key ribosomal protein with eCLIP (enhanced crosslinking and immunoprecipitation). The ability to quantitate the ribosome occupancy on transcripts can yield insight into translational efficiency and also provides a means to measure the effect of drug treatment on protein translation.

Technology Highlights

Quantify ribosome occupancy

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Differential expression analysis

RESOURCES

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Services Tailored to Your Experiment

We offer all-inclusive sample processing with expert library preparation and bioinformatic analysis.