A simplified approach to count ribosome- associated polyadenlyated transcripts
Technology Overview
eRibo Count is a method that quantitates ribosome association transcriptome-wide by targeting a key ribosomal protein with CLIP (crosslinking and immunoprecipitation). eRibo Count is a streamlined CLIP protocol by which libraries of the sample’s polyadenylated transcriptome and translatome can be generated within two days. The ability to quantitate ribosome occupancy on transcripts can yield insight into translational efficiency. This provides a means to measure differences in expression between samples, e.g. normal vs. disease tissue or drug treated vs. untreated samples. eRibo Count quantitates ribosome association transcriptome-wide by targeting a key ribosomal protein, and supplies two comprehensive data sets.
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Simple quantitation of ribosome occupancy changes
- Reduced sequencing depth required with reads enriched at ribosome associated transcript ends
- eRibo Count can quantitate changes in ribosome occupancy in response to treatment that would otherwise be missed with RNA-Seq alone
Compute translational efficiency
- eRibo Count can measure changes in ribosome association on transcripts. Ribosome occupancy (RO) is a ratio of ribosome IP to RNA-Seq thus normalizing to changes in transcript levels
- Gain a deeper understanding of how regulation and mis-regulation of translation impact physiology and disease progression
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