AURORA 3'
Rapid, easy, and efficient method to quantify ribosome occupancy. Obtain gene-level expression data.
Technology Highlights
- Detect changes in translation that would be missed with RNA-Seq alone
- Rapid, easy protocol
- Gene-level expression data for gene counting
AURORA 3' IS NOW AVAILABLE FOR EARLY ACCESS
Figure 1. Torin 1 is a known translation inhibitor. (A) AURORA 3′ reads mapped to the EEF1A1 gene before (left side) and after (right side) Torin treatment. RNA-Seq levels are constant while signal from the IP decreases upon treatment. (B) MA plots of genes. Blue dots represent significant differences in response to Torin 1.
AURORA Total
Obtain ribosome occupancy data and RNA-Seq data across the entire transcriptome. Identify splice variants and calculate ribosome occupancy.
Technology Highlights
- Transcriptome-wide detection of ribosome associated transcripts
- Quantify changes in ribosome occupancy
- Modified eCLIP technology with no gel or ultracentrifugation steps required
- Generates libraries with long insert lengths to identify splicing events missed by ribosome profiling
AURORA Total is now available for early access
Figure 2. Ribosome occupancy, calculated as AURORA Total/RNA-seq (aka translational efficiency or TE), reports the expected decrease in ribosome occupancy of RNAs containing TOP- and TOP-like motifs after Torin 1 treatment.