AURORA 3'

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Rapid, easy, and efficient method to quantify ribosome occupancy. Obtain gene-level expression data.

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Figure 1. Torin 1 is a known translation inhibitor. (A) AURORA 3′ reads mapped to the EEF1A1 gene before (left side) and after (right side) Torin treatment. RNA-Seq levels are constant while signal from the IP decreases upon treatment. (B) MA plots of genes. Blue dots represent significant differences in response to Torin 1.

AURORA Total

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Obtain ribosome occupancy data and RNA-Seq data across the entire transcriptome. Identify splice variants and calculate ribosome occupancy.

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Figure 2. Ribosome occupancy, calculated as AURORA Total/RNA-seq (aka translational efficiency or TE), reports the expected decrease in ribosome occupancy of RNAs containing TOP- and TOP-like motifs after Torin 1 treatment.

AURORA RiboPro

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