Figure 1. Torin 1 is a known translation inhibitor. (A) AURORA 3′ reads mapped to the EEF1A1 gene before (left side) and after (right side) Torin treatment. RNA-Seq levels are constant while signal from the IP decreases upon treatment. (B) MA plots of genes. Blue dots represent significant differences in response to Torin 1.
AURORA Total
Obtain ribosome occupancy data and RNA-Seq data across the entire transcriptome. Identify splice variants and calculate ribosome occupancy.
Technology Highlights
Transcriptome-wide detection of ribosome associated transcripts
Quantify changes in ribosome occupancy
Modified eCLIP technology with no gel or ultracentrifugation steps required
Generates libraries with long insert lengths to identify splicing events missed by ribosome profiling
Figure 2.Ribosome occupancy, calculated as AURORA Total/RNA-seq (aka translational efficiency or TE), reports the expected decrease in ribosome occupancy of RNAs containing TOP- and TOP-like motifs after Torin 1 treatment.