ADAR1-eCLIP
Robust identification of isoform-specific
ADAR1 binding sites transcriptome-wide
TECHNOLOGY OVERVIEW
Adenosine deaminase acting on RNA (ADAR1) is an RNA editing enzyme that preferentially binds double-stranded RNA and catalyzes the site-specific deamination of adenosine, resulting in the conversion of adenosine to inosine. There are two ADAR1 isoforms, a constitutively expressed p110 and an interferon induced p150. The ADAR1-eCLIP technology maps the RNA binding sites of ADAR1 transcriptome-wide and in an isoform-specific manner utilizing isoform specific antibodies directed against ADAR1 to immunoprecipitate cross-linked target RNAs.
Technology Highlights
Reproducible and high-confidence identification of ADAR1 targets transcriptome-wide
- ADAR1-eCLIP cells identifies thousands ADAR1 binding sites primarily in intronic regions and enriched for A-to-I editing
- Isoform-specific profiling of ADAR1 p110 and p150 resolves different RNA binding preferences of the isoforms
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High Resolution ADAR1 binding sites
- ADAR1-eCLIP read densities correspond to structural features of ADAR1 target RNAs
- ADAR1 binding sites are identified in regions expected to fold into dsRNA structures
RESOURCES
Services Tailored to Your Experiment
We offer all-inclusive sample processing with expert library preparation and bioinformatic analysis.
