Application

ASO

Developing an Antisense Oligonucleotide (ASO) to degrade transcripts, alter splicing, or alter translation? Our comprehensive solutions will help you succeed.

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Service Solutions

Use RNA structure to guide design with eSHAPE

  • Focus screening by identifying regions with appropriate accessibility
  • Plan for cellular effects by determining if regions change accessibility in the cellular environment compared to in vitro

Confirm transcription and translation effects with eRibo Pro

  • Confirm decreased translation after ASO treatment
  • Identify translation specific effects such as stalls
  • Screen for off target effects in both the transcriptome and translatome

Determine changes in protein binding with RBP-eCLIP

  • Validate which RBPs bind to the target transcript
  • Look for changes in the binding of known protein cofactors such as splicing factors

Find novel UTRs to target with End-Seq

  • Map the UTR landscape in your sample to find novel regions to target
  • Determine if UTR usage is affected by ASO treatment

Screen for existing miRNA regulators with miR-eCLIP

  • Find miRNAs that could compete with your ASO by binding to the region of interest
  • Obtain a fuller picture of how your target is regulated

Use RNA structure to guide design with eSHAPE

  • Focus screening by identifying regions with appropriate accessibility
  • Plan for cellular effects by determining if regions change accessibility in the cellular environment compared to in vitro

Confirm transcription and translation effects with eRibo Pro

  • Confirm decreased translation after ASO treatment
  • Identify translation specific effects such as stalls
  • Screen for off target effects in both the transcriptome and translatome

Determine changes in protein binding with RBP-eCLIP

  • Validate which RBPs bind to the target transcript
  • Look for changes in the binding of known protein cofactors such as splicing factors

Find novel UTRs to target with End-Seq

  • Map the UTR landscape in your sample to find novel regions to target
  • Determine if UTR usage is affected by ASO treatment

Screen for existing miRNA regulators with miR-eCLIP

  • Find miRNAs that could compete with your ASO by binding to the region of interest
  • Obtain a fuller picture of how your target is regulated