Description
Highlights
Deep Coverage from eSHAPE single & total kits
Extremely deep RNA coverage >10,000X with minimal sequencing depth
Reactivities of high sensitivity & specificity
Reactivity values are predictive of paired and unpaired base status in an RNA secondary structure fold
Detect structural changes due to sequence variants
Compare reactivities of similar RNAs to identify regions of RNA folding variability
eSHAPE single Kit
An easy and fast protocol that allows the user to obtain in vitro SHAPE data through NAI structure probing of an IVT RNA
eSHAPE total Kit
An easy and fast protocol that allows the user to obtain in vitro SHAPE data through structure probing of total RNA, or processing in cellulo probed total RNA to gain in cellulo SHAPE data.
