The eSHAPE Service
Turnaround Time
Service: 6 – 8 Weeks (Project Dependent)
Starting Material
1ug in vitro transcribed RNA
Start your eSHAPE Service Project
Connect With An Eclipsebio RNA Expert
Bioinformatics
Our Comprehensive Data Analysis Package
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
RNA ALIGNMENTS | BAM
Reads aligned to the RNA of interest, and PCR deduplicated for additional downstream analysis
MUTATION RATES | TSV
Mutation rates for each NAI and DMSO sample per RNA position for reactivity calculations
eSHAPE REACTIVITY | BEDGRAPH, SHAPE
IQR normalized reactivity score at each position of the RNA of interest with >1000x coverage for visualization in a genome viewer such as IGV or for use as input into the RNAStructure algorithm to guide fold predictions
eSHAPE SUMMARY REPORT | HTML
Report of eSHAPE results with interactive tables and plots for publication ready graphics. Includes QC metrics, coverage, mutation rates and reactivities for the RNA of interest
REACTIVITY COMPARISON REPORT | HTML
Report of differential reactivities for experiments with multiple conditions or RNAs for comparison. Includes interactive plots and results table denoting significantly different positions with p-values
