All-Inclusive Service

5′ End-Seq

Reveal 5′ UTR Landscape and Transcription Start Sites Genome-wide

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The 5′ End-Seq Service

Turnaround Time
Service: 4 – 6 Weeks (Project Dependent)

Starting Material
10 µg Total RNA; RIN ≥ 7; Non-Crosslinked
Minimum Batch: 8 Samples

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Connect With An Eclipsebio RNA Expert

Bioinformatics

Our Comprehensive Data Analysis Package

Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), rat (rn6), or c. elegans (ce11). Contact us for alternate species including viral genomes.

RAW SEQUENCING DATA | FASTQ

Reads directly off sequencer and demultiplexed per sample for data back-up and publication

GENOME ALIGNMENTS | BAM

Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis

COVERAGE TRACKS | BIGWIG

Normalized read end coverage on positive and negative strands for visualization in a genome viewer such as IGV

SINGLE NUCLEOTIDE 5′ END CALLS | BED

Genomic single nucleotide positions scored as putative 5’ transcript ends for 5’UTR exploration

5′ End-Seq SUMMARY REPORT | HTML

Report with interactive tables and plots for publication ready graphics. Includes QC metrics and 5’ end enrichment

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