microRNA -mRNA Chimeric eCLIP

Identify microRNA-mRNA targets

microRNAs are short regulatory RNAs that repress gene expression through binding of complementary sequences in mRNA 3′ Untranslated Regions

Modification of eCLIP enables ligation of microRNAs with mRNA targets to create chimeric fragments that unambiguously map individual microRNA binding sites with high resolution

Full-service: from your sample to analyzed data

 

Chimeric eCLIP service includes:

Chimeric eCLIP assay

  • Sequencing library preparation
  • High-throughput sequencing

Experimental quality assays

  • Library yield metrics

Sequencing analysis

  • Read processing and mapping
  • Peak identification & normalization against input control

Analysis summary report

  • Read-level metrics including % mapping & PCR duplication rate
  • Peak meta-gene profiles
  • Basic motif analysis

Contact us for additional information

Please contact us to receive a quote for custom services or to ask any questions you may have. A brief description of your desired experiment (number of samples, custom versus commercial antibody) will enable us to better respond to your queries.