m6A sites can be identified with eCLIP

  • N6-methyladenosine (m6A) is an abundant modification in mRNA.
  • m6A has diverse molecular functions- modulates many mRNA processes, as mRNA export, stability, translation efficiency, etc.
  • m6A-eCLIP is a modified version of eCLIP (Van Nostrand et al. Nature Methods, 2016) using a validated m6A antibody.
  • m6A-eCLIP uses 40-fold less mRNA and requires fewer PCR cycles than previously published methods.
  • m6A-eCLIP captures m6A motif and enrichment at stop codons of genes.

Rigorous in-line validation

1.Library yield and size distribution confirmed by Tapestation and qPCR

2.Generate complex libraries with high yields.

3.Sequencing to yield 20 million reads

4.Identify m6a sites with a single nucleoside resolution.

RNA-seq control significantly improves signal/noise to identify true modification targets

Contact us for additional information

Please contact us to receive a quote for custom services or to ask any questions you may have. A brief description of your desired experiment (number of samples, custom versus commercial antibody) will enable us to better respond to your queries.