m6A-eCLIP

Quick, efficient and precise identification
of m6A modification sites

N6 methyladenosine (m6A) is the most common internal epitranscriptomic RNA modification
Our m6A-eCLIP Service and Kit provide a robust method to obtain an enriched m6A library in one week

High reproducibility

Biological replicates are well correlated

Single Nucleotide Motif

Most common DRACH-6 mer in 400ng m6A-eCLIP shows enrichment at -3 position relative to read starts

Unbiased Sensitivity

m6A-eCLIP captures the DRACH motif and enrichment at stop codons and 3′ UTRs of genes

High throughput

Uses 40-fold less mRNA and requires fewer PCR cycles than previously published methods

High Accuracy

RNA-seq control significantly improves signal/noise to identify true modification sites

A validated m6A antibody is cross-linked to mRNA sequences, and a cDNA library is prepared and sequenced

Eclipse Bio’s m6A-eCLIP Service and Kit

Full-service: from total RNA to analyzed data

m6A eCLIP service includes:

m6A eCLIP assay

  • Immunoprecipitation with Eclipse m6A validated antibody
  • Sequencing library preparation
  • High-throughput sequencing

Experimental quality assays

  • Library yield metrics

Sequencing analysis

  • Read processing and mapping
  • Peak identification & normalization against input control

Analysis summary report

  • Read-level metrics including % mapping & PCR duplication rate
  • Peak meta-gene profiles
  • Basic motif analysis

Robust and easy to use m6A-eCLIP Kit

m6A eCLIP Kit includes:

  • Reagents for 8 samples (8 m6A-IPs and 8 Inputs)
  • m6A-eCLIP validated antibody
  • All reagents, enzymes, and oligonucleotides necessary to make m6A libraries

Contact us for high throughput variants or other customized needs

Contact us for additional information

Please contact us to receive a quote for custom services or to ask any questions you may have. A brief description of your desired experiment (number of samples, custom versus commercial antibody) will enable us to better respond to your queries.