Identify microRNA-mRNA targets
microRNAs are short regulatory RNAs that repress gene expression through binding of complementary sequences in mRNA 3′ Untranslated Regions
Modification of eCLIP enables ligation of microRNAs with mRNA targets to create chimeric fragments that unambiguously map individual microRNA binding sites with high resolution
Full-service: from your sample to analyzed data
Chimeric eCLIP service includes:
Chimeric eCLIP assay
- Sequencing library preparation
- High-throughput sequencing
Experimental quality assays
- Library yield metrics
Sequencing analysis
- Read processing and mapping
- Peak identification & normalization against input control
Analysis summary report
- Read-level metrics including % mapping & PCR duplication rate
- Peak meta-gene profiles
- Basic motif analysis
