Next Tuesday, October 6 2020, we will be the Silver Sponsor of “The Complex Life of RNA” Webinar, hosted by EMBO | EMBL Symposia. Together with our guest speaker Dr. Allison Porman Swain from the University of Colorado, we will be talking about the eCLIP platform and how m6A regulates lncRNA HOTAIR in breast cancer.
Join us at 16:00-17:00 (CEST) or 10:00-11:00am (EST)!
The eCLIP platform: A powerful technology to study RNA binding protein interactions, microRNA targets and RNA modifications
Presenter: Dr. Sergei Manakov (Eclipse Bioinnovations)
Abstract: Eclipse Bio has quickly become a leader in the new frontier of RNA genomics. Our eCLIP technology platform enables all scientists to understand post-transcriptional regulation by mapping targets for RNA binding proteins (RBPs), RNA modifications, and non-coding RNAs such as miRNAs and lncRNAs.
eCLIP technology was used to profile targets of 150 RBPs for the ENCODE project (Encyclopedia of DNA Elements), an international collaboration that aims to build a comprehensive parts list to understand how genes are controlled. The recently released paper entitled, “A large-scale binding and functional map of human RNA-binding proteins,” is available open access on Nature as part of a special package of 10 studies from ENCODE Consortium.
Sergei Manakov, Principal Scientist Bioinformatics at Eclipse Bioinnovations, will present a detailed overview of the eCLIP technology and its applications for robust and precise mapping of RNA binding proteins, m6A modifications and miRNA-mRNA targets. Eclipse Bio enables researchers in all research fields (including cancer, CNS, genetic disease and virology) to understand RNA regulation in diseases, cells, and model organisms.
m6A regulates lncRNA HOTAIR in breast cancer
Guest Speaker: Dr. Allison Swain (University of Colorado Denver)
Abstract: N6-methyladenosine (m6A) is one of the most abundant RNA modifications that has important roles in normal and cancer biology, but knowledge of its function on long noncoding RNAs (lncRNAs) remains limited. To investigate a potential role for m6A on the lncRNA HOTAIR, we mapped m6A at single nucleotide sites in HOTAIR-expressing breast cancer cells using m6A enhanced cross-linking immunoprecipitation (meCLIP) (Van Nostrand et. al. Nature Methods, 2016). We identify at least 11 m6A modified adenosine residues within HOTAIR and show that modification of HOTAIR is mediated by the m6A transferase METTL3/14 complex. This and additional data to be presented in this seminar suggest a mechanism whereby m6A is important in regulating the function of HOTAIR via mediating interaction of YTHDC1 with specific m6A sites.