Eclipse Bio would like to bring to your attention that a modified version of our “eCLIP kit” has been recently launched and renamed to “RBP-eCLIP”. The former eCLIP kit, (cat # ECK001) has been permanently DISCONTINUED. The latest version of the RBP-eCLIP kit includes two additional quality control steps to help facilitate experimental design and maximize data quality.

1. Change: RNA quality and quantity assessment prior to overnight IP on Day 1

a. Reason: This step allows for RNA fragmentation optimization for a variety of different types of starting material (cells or tissue) and factors in the RNA integrity (RIN). Supplemental material available upon request

2. Change: RNA imaging blot on Day 2

a. Reason: Imaging RNA post IP allows for confirmation of successful crosslinking, validates antibody efficiency and confirms protein of interest as a bona fide RBP.

Additional optimization for reagents volumes and procedural steps have been incorporated.

For your convenience, we have provided the links to both versions of protocols below.
Updated Version: https://eclipsebio.com/wp-content/uploads/protocols/EclipseBio_RBP-eCLIP_Protocol.pdf

Discontinued Version: https://eclipsebio.com/wp-content/uploads/protocols/EclipseBio_eCLIP_Protocol_v1.01R.pdf

If you have any questions or concerns, please do not hesitate to reach out to us at techsupport@eclipsebio.com

Sincerely,

The Eclipse Bio Team