RNA Genomics

Services

Whether you are conducting exploratory research projects, characterizing new RNA drug targets, or developing the next generation of RNA therapeutics, we offer RNA genomics solutions for your needs.

Services

RBP-eCLIP

RBP-eCLIP (Enhanced crosslinking and immunoprecipitation, eCLIP) followed by high-throughput sequencing was developed to provide a robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide.

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Service: 6-8 Weeks (Project Dependent)

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  • Cells: 20 M; UV Crosslinked
    Tissue: 80 mg; NOT Crosslinked; Flash Frozen
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miR-eCLIP

miR-eCLIP enables precise mapping of direct miRNA-mRNA interactions transcriptome wide. In contrast to alternative techniques that provide indirect methods to map miRNA targets (i.e., computational algorithms or over-expression/knockdown experiments), miR-eCLIP identifies direct in vivo miRNA targets using AGO2 immunoprecipitation, RNA-RNA ligation, and high-throughput sequencing (similar to methods such as CLASH or CLEAR-CLIP).

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6-8 Weeks (Project Dependent)
+2 Weeks for Enrichment

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  • Eclipsebio provided AGO2 antibody
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  • Cells: 20 M; UV Crosslinked; Mouse, Human
    Tissue: 80 mg; NOT Crosslinked; Human, Mouse, Rat, C. elegans
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m6A-eCLIP

m6A-eCLIP robustly profiles m6A modification sites with single nucleotide resolution transcriptome-wide. In comparison to alternate approaches, m6A-eCLIP requires 100-fold less starting RNA material for m6A profiling.

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Service: 6-8 Weeks (Project Dependent)

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  • Eclipsebio provided m6A antibody
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  • 50ug Total RNA; RIN >7
    Isolated from Cells or Tissues
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eRibo Pro

eRibo Pro is a proprietary ribosome profiling service that streamlines the ribosomal profiling process, from sample processing to sequencing and data analysis. The comprehensive service includes mapping of ribosome protected fragments (RPFs) and identification of differentiallv transcribed and translated genes with interactive reports that allow researchers to make data driven insights.

Eclipsebio is sole licensee of the Ribo-seq method covered by U.S. Patent Numbers 8,486,865, 9,284,547 and 9,677,068.

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Service: 6 – 8 Weeks (Project Dependent)

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  • 5M Cells
  • Starting from 70% – 80% Confluent Plates
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eSHAPE Single RNA

eSHAPE is a proprietary method to obtain RNA structure probing data for in vitro transcribed RNA formulations. In vitro transcribed or synthetic RNAs are used as input for RNA structure probing with the NAI reagent. eSHAPE single RNA is available as a kit for end user completion, and as a full service offering with results delivered as a data package containing RNA structure information as reactivity values.

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Service: 6 – 8 Weeks (Project Dependent)

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  • 1ug in vitro transcribed RNA
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5′ End-Seq

5′ End-Seq can facilitate precise UTR identification for RNA therapeutics targeting and can be used as a tool for identifying UTR biomarkers associated with disease. 5′ End-Seq can quantify the relative usage of TSS across samples and indicates transcript isoform presence in annotated transcriptomes. Defining the 5′ UTR with End-Seq can help predict binding factor motifs more reliably

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Service: 4 – 6 Weeks (Project Dependent)

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  • 10 µg Total RNA; RIN ≥ 7; Non-Crosslinked
  • Minimum Batch: 8 Samples
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3′ End-Seq

3′ End-Seq can facilitate precise UTR identification for RNA therapeutics targeting and can be used as a tool for identifying UTR biomarkers associated with disease. 3′ End-Seq can quantify the relative usage of PAS across samples and indicate transcript isoform presence in annotated transcriptomes. Defining the 3′ UTR with End-Seq helps predict binding factor motifs more reliably.

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Service: 4 – 6 Weeks (Project Dependent)

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  • 10 µg Total RNA; RIN ≥ 3; Non-Crosslinked
  • Minimum Batch: 8 Samples
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Learn More About Our RNA Genomics Technologies

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