Quick, efficient and precise identification
of m6A modification sites
N6 methyladenosine (m6A) is the most common internal epitranscriptomic RNA modification
Our m6A-eCLIP Service and Kit provide a robust method to obtain an enriched m6A library in one week
High reproducibility
Biological replicates are well correlated
Single Nucleotide Motif
Most common DRACH-6 mer in 400ng m6A-eCLIP shows enrichment at -3 position relative to read starts
Unbiased Sensitivity
m6A-eCLIP captures the DRACH motif and enrichment at stop codons and 3′ UTRs of genes
High throughput
Uses 40-fold less mRNA and requires fewer PCR cycles than previously published methods
High Accuracy
RNA-seq control significantly improves signal/noise to identify true modification sites
A validated m6A antibody is cross-linked to mRNA sequences, and a cDNA library is prepared and sequenced
Eclipse Bio’s m6A-eCLIP Service and Kit
Full-service: from total RNA to analyzed data
m6A eCLIP service includes:
m6A eCLIP assay
- Immunoprecipitation with Eclipse m6A validated antibody
- Sequencing library preparation
- High-throughput sequencing
Experimental quality assays
- Library yield metrics
Sequencing analysis
- Read processing and mapping
- Peak identification & normalization against input control
Analysis summary report
- Read-level metrics including % mapping & PCR duplication rate
- Peak meta-gene profiles
- Basic motif analysis
Robust and easy to use m6A-eCLIP Kit
m6A eCLIP Kit includes:
- Reagents for 8 samples (8 m6A-IPs and 8 Inputs)
- m6A-eCLIP validated antibody
- All reagents, enzymes, and oligonucleotides necessary to make m6A libraries
Contact us for high throughput variants or other customized needs