microRNAs are short regulatory RNAs that repress gene expression through binding of complementary sequences in mRNA 3′ Untranslated Regions
Modification of eCLIP enables ligation of microRNAs with mRNA targets to create chimeric fragments that unambiguously map individual microRNA binding sites with high resolution
miR-eCLIP directly maps individual microRNA targets
Standard Ago2 eCLIP identifies microRNA landing sites
Whole-transcriptome miR-eCLIP maps targets of all microRNAs
miR-specific library preparation options enable deep profiling of an individual microRNA of interest
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microRNA targets can be identified with high specificity through Ago2 miR-eCLIP.
